《植物生理学报》 2017, 53(1): 89-95

水杨酸(SA)通过抑制拟南芥中PIN3的表达影响顶端弯钩的形成和下胚轴对向光性的响应

王愿, 唐玉梅, 王晓坤, 戈海曼, 杨磊^*

南京大学生命科学学院, 南京210093

通信作者：杨；E-mail: leiyang@nju.edu.cn

摘　要：

水杨酸(SA)是植物体内一种很重要的植物激素, 它能够调控植物对逆境的响应和影响植物的很多生长发育过程, 但是其对双子叶植物顶端弯钩的发育和下胚轴对单侧光的响应的影响, 从来没有被报道。本文以模式植物拟南芥为材料, 用高浓度外源的SA处理后, 我们发现SA会抑制拟南芥黄化苗顶端弯钩的形成, 而且导致下胚轴向光生长存在缺陷。进一步研究发现, SA会导致生长素在顶端弯钩内侧的分布减弱, 在单侧光刺激下生长素向背光侧的极性转运过程也受到了抑制。结合我们的实验结果和生长素输出转运体PIN3的生理功能, 我们认为SA通过抑制PIN3的表达来影响由生长素所介导的顶端弯钩的发育和下胚轴对单侧光的响应过程。这一发现, 有助于加强我们对SA在植物体内所发挥的生理作用的了解。

关键词：顶端弯钩; 向光性; 生长素; 水杨酸

收稿：2016-11-01   修定：2016-12-02

资助：江苏省自然科学基金青年基金(BK2012306)。

Salicylic acid (SA) inhibits the expression of auxin efflux transporter PIN3 to control the establishment of apical hook and phototropic response of hypocotyl

WANG Yuan, TANG Yu-Mei, WANG Xiao-Kun, GE Hai-Man, YANG Lei^*

School of Life Sciences, Nanjing University, Nanjing 210093, China

Corresponding author: YANG Lei; E-mail: leiyang@nju.edu.cn

Abstract:

Salicylic acid (SA) plays an important role in multiple physiological processes including response to environment stresses and many development processes. It’s still not clear whether SA affects the development of apical hook and the response to unilateral light. In this study, we used the model plant Arabidopsis thaliana to examine the effect of high level exogenous SA on the development of apical hook and the response to unilateral light. We found that exogenous SA treatment could inhibit the formation of apical hook of A. thaliana, and cause defect in the phototropic growth of hypocotyl. We further found that the auxin accumulation in the concave side of apical hook was decreased and the auxin can not move toward to the side of backlight upon treatment with SA. Based on the function of auxin efflux transporter PIN3 in Arabidopsis, a clear explanation for our findings is that SA inhibits the expression of auxin efflux transporter PIN3 to control the establishment of apical hook and phototropic response of hypocotyl. Our results provide insights on the physiological role of SA in plants.

Key words: apical hook; phototropic; auxin; salicylic acid